INTERNATIONAL CONGRESS OF
CancerGenetics
Investigating the interaction between the WNT2 gene as a potential biomarker and its associated miRNA in colorectal cancer through bioinformatics analysis
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mehdi roshanian bakhsh,1,* abbas zabihi,2
1. دانشگاه علوم پزشکی اسداباد
2. Department of Biology, faculty of basic sciences, Islamic Azad University, rasht, Iran.
- Introduction: Colorectal cancer is the third most common cancer and the second leading cause of cancer related deaths worldwide, with an estimated 1.8 million new cases and approximately 881,000 deaths in 2018. Bioinformatics analyses were conducted to identify genes differentially expressed in carcinogenesis and progression of CRC.
- Methods: The GSE156355 dataset was retrieved from GEO to identify the differentially expressed genes (DEGs) between 6 tumor and 6 healthy samples in a colorectal cancer microarray experiment. The GEO2R was used on DEGs to compare CRC samples with noncancerous samples. The adjusted P-value < 0.01 and log2 fold change > 4 were considered as statistical significances. Gene functional classification was performed using DAVID, and pathway analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG). miRDB was queried to identify microRNA with WNT2, and miRWalk was used to investigate interactions between the selected miR and associated genes.
- Results: A total of 33 DEGs were identified. DAVID analysis indicated a role for WNT2 in the positive regulation of cell population proliferation in colorectal cancer. KEGG pathway analysis showed that WNT2 is involved in the Wnt signaling pathway, which regulates the cell cycle; inhibition of WNT2 may result in cell cycle arrest in colorectal cancer cells. Our analysis also suggested that has-mir-4310 may suppress WNT2 expression, potentially leading to cell cycle arrest in CRC cells.
- Conclusion: Based on bioinformatics analyses, WNT2 may serve as a biomarker and contribut to Wnt signaling in colorectal cancer. hsa-mir-4310 could be explored as a therapeutic approach to downregulate WNT2 and inhibit cell proliferation in these patients.
- Keywords: has-mir-4310, GEO, cell cycle arrest, DEGs, therapeutic strategy
