INTERNATIONAL CONGRESS OF
CancerGenetics
The effect of extracellular polysaccharides (EPS) from Lactobacillus casei on advanced liver cancer
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milad saber,1,* Razieh Nazari,2 majid nejati,3
1. Azad university
2. Azad university
3. medical kashan university
- Introduction: Liver cancer is the third leading cause of cancer death in the world. Current treatments include surgery, chemotherapy, and targeted drugs, but they are often associated with disease recurrence and severe side effects. In the meantime, attention has been paid to probiotic metabolites such as EPS. EPS produced by L. casei, in addition to its antioxidant and anti-inflammatory properties, also has the ability to inhibit the growth of tumor cells.
- Methods: Samples: Human hepatoma cells (HepG2). Intervention: Treatment with different concentrations of purified EPS from L. casei (50–500 μg/ml). Experiments: - MTT assay to measure cell viability. - Annexin V/PI staining to assess apoptosis. - ELISA assay to determine inflammatory cytokines (TNF-α, IL-6). - Examination of Bax, Bcl-2 and STAT3 pathway gene expression by RT-PCR and Western blot.
- Results: The findings indicate that L. casei EPS can act both directly by inhibiting HCC cell growth and inducing apoptosis, and indirectly by reducing inflammation and improving liver function. The use of these compounds, especially in the form of fermented foods (such as probiotic yogurts), can be considered as an adjunct strategy in the prevention or complementary treatment of liver cancer.
- Conclusion: a
- Keywords: Lactobacillus casei, (EPS), liver cancer
