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  • Accepted papers of the First International Congress of CancerGenetics
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  • Bioinformatics Analysis Identifies Shared lncRNAs Associated with CDH5 and PTPRB in the Adherens Junction Pathway of Lung Adenocarcinoma

  • Arsham Azari Dehkordi,1,* Pegah Bozorgzad Moghim,2 Maryam Sadat Sajadi Javan,3 Shiva Vaheb Hosseinabadi,4 Mansoureh Azadeh,5 Sara Amini,6
    1. Zist Fanavari Novin Biotechnology Institute
    2. Zist Fanavari Novin Biotechnology Institute
    3. Zist Fanavari Novin Biotechnology Institute
    4. Zist Fanavari Novin Biotechnology Institute
    5. Zist Fanavari Novin Biotechnology Institute
    6. Zist Fanavari Novin Biotechnology Institute


  • Introduction: Despite the progress of medicine, lung cancer remains a public health issue and the most common reason for cancer death globally. Its most common subtype is lung adenocarcinoma (LUAD). The metastasis and pathogenesis of LUAD remain unclear, at least in part because it has been challenging to reproduce disease progression successfully. Aberrantly expressed long non-coding RNAs (lncRNAs) play a role in many human diseases, including cancer. This study aimed at discovering potential LUAD biomarkers through the use of the application of bioinformatics tools.
  • Methods: LUAD gene expression was analyzed using the GSE118370 microarray dataset of GEO2R, and the dysregulated genes were selected with extreme dysregulation. Protein–protein interactions of the selected genes were determined by STRING, and the pathways that were engaged by KEGG were explored. Gene expression value and correlation were then confirmed by GEPIA2 and ENCORI. Target miRNAs overlapping were downloaded from miRTarBase, TargetScan, and miRDB, and their interaction was observed with Cytoscape. The overlapping lncRNAs were last found by utilizing LncBase (DIANA) and were observed using Cytoscape for network.
  • Results: Among two genes found out, CDH5 (logFC = –2.495, adj.p.val = 0.000946) and PTPRB (logFC = –2.849, adj.p.val = 0.000946), both were significantly downregulated in LUAD patients. Both the genes were highly correlated with each other, correlation coefficient R = 0.9. Both CDH5 and PTPRB were found to belong to the Adherens junction signaling pathway (KEGG: hsa04520); they are within the same regulation pathway. Two miRNAs, miR-125a-5p and miR-125b-5p, were found to target CDH5, and five miRNAs were found to be targeted by PTPRB. Furthermore, nine lncRNAs were both associated with the two genes, including SNHG12, TP53TG1, LINC-ROR, SNHG14, HMMR-AS1, GAS5, MALAT1, HOTTIP, and CYTOR, reflecting shared regulation mechanisms.
  • Conclusion: Interference of the CDH5 and PTPRB involved Adherens junction pathway has the ability to interfere with cell–cell adhesion and promote cellular motility, leading to metastasis and cancer in LUAD. Shared lncRNAs associated with the two genes reveal a novel regulatory pathway, casting new light on molecular mechanisms and potential therapeutic targets for diagnostic and treatment intervention.
  • Keywords: Lung Adenocarcinoma, CDH5, PTPRB, Long Non-Coding RNA, Adherens Junction Pathway

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