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INTERNATIONAL CONGRESS OF

CancerGenetics

• Peripheral blood IGF2 hypomethylation as a promising non-invasive indicator of colorectal cancer prognosis

• Yousef Mohammadi,¹ Elham Zare ,² Ali Beheshti Namdar,^3,*
  1. Student Research Committee, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
  2. Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  3. Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  
  
  
• Introduction: Conventional colorectal cancer (CRC) screening methods are hindered by invasiveness and insufficient accuracy, emphasizing the need for non-invasive biomarkers. DNA methylation alterations occur early in tumor development, and hypomethylation of the IGF2 DMR0 region disrupts normal imprinting, leading to IGF2 overexpression and subsequent tumor progression. Given that peripheral blood mononuclear cells (PBMCs) not only provide an indirect source of biomarkers but also mirror the methylation profile of tumor cells, we evaluated IGF2 DMR0 methylation in PBMCs from Iranian CRC patients and healthy controls.
• Methods: PBMCs were isolated from peripheral blood specimens obtained from 62 sporadic CRC participants and 62 healthy individuals. The quantitative methylation analysis of the IGF2 DMR0 region was conducted using the methylation quantification endonuclease-resistant DNA (MethyQESD) method.
• Results: The methylation level of IGF2 DMR0 was found to be lower in CRC patients (21.44 ± 16.77%) compared to controls (24.63 ± 16.27%); however, this observed disparity did not reach statistical significance (P: 0.286). When stratified by tumor stage, early-stage tumors (Stages I and II) showed significantly higher methylation levels than advanced-stage tumors (Stages III and IV) (P< 0.001). Furthermore, Pearson correlation analysis discovered a significant negative association between tumor size and methylation level (r:  -0.624, P< 0.001). No remarkable correlations were identified between methylation levels and other parameters such as gender, age, tumor location, or smoking status (P> 0.05).
• Conclusion: Our findings proposed that hypomethylation of the IGF2 DMR0 region is meaningfully linked with tumor progression and growth, underscoring its potential as a non-invasive prognostic biomarker for CRC.
• Keywords: Colorectal cancer, IGF2 gene, Methylation, Biomarker