INTERNATIONAL CONGRESS OF
CancerGenetics
Genomic profiling of TMX2 reveals functional variants driving Gliobblastoma
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pardis karimian,1,* Aida sattari,2 Hamid Reza Roshani Dastgerdi,3 Shiva vaheb hosseinabadi,4 aysa nasiri,5 Forough jannesari,6
1. Zist Fanavari Novin
2. Zist fanavari novin
3. Zist fanavari novin
4. Zist fanavari novin
5. zist fanavari novin
6. zist fanavari novin
- Introduction: Introduction: Glioblastoma Multiforme (GBM) is one of the most significant brain tumors in adults, characterized as a rapidly growing malignant tumor. It primarily affects individuals aged 45 to 70, with about 15% of all brain tumors attributed to GBM.
- Methods: Methods: Microarray analysis was performed on GSE165813 from GEO databases. The TMX2 gene was identified as significantly expressed, and results were confirmed using GEPIA2 databases. The signaling pathways involving TMX2 were examined through REACTOME and KEGG databases. An analysis of related SNPs from SIFT, miRNA SNP, and dbSNP databases led to the selection of rs1486249546 in the 3' UTR and rs367990143 in the coding sequence. Protein-protein interactions were analyzed using the STRING database, and mRNA-miRNA interactions were assessed through Mirwalk.
- Results: Results: The deleterious SNP rs367990143 indicated a mutation from Glycine to Arginine at position 56, with the larger mutant residue. The wild-type residue has a neutral charge, while the mutant residue is positively charged and less hydrophobic. Hsa-miR-5p was identified as influencing the 3' UTR, and KCNQ10T1 was selected from the lncRResearch database, associating with TMX2.
- Conclusion: Conclusion: The results of this study indicate a significant increase in TMX2 gene expression in Glioblastoma Multiforme cancer following microarray analysis.
- Keywords: Keyword:TMX2 gene, Microarray analysis, rs367990143 , rs1486249546, lncRNA KCNQ10T1
