Role of CRISPR/Cas9 Gene Editing in Targeting CXCR4, CD133 and EpCAM to Inhibit ‎Cell Adhesion and Metastasis in Colorectal Cancer

بازنشر

Role of CRISPR/Cas9 Gene Editing in Targeting CXCR4, CD133 and EpCAM to Inhibit ‎Cell Adhesion and Metastasis in Colorectal Cancer
Amir Mahdi Alizadeh Langehibz,¹ zohreh shadmehr,^2,*
1. B.Sc. Student of Animal Biology, Department of Biology, Tehran Central Branch, Islamic Azad ‎University, Tehran, Iran
2. Ph.D. Candidate in Microbiology, Department of Biology, Science and Research Branch, Islamic ‎Azad University, Tehran, Iran
Introduction: Colorectal cancer (CRC) is a major cause of cancer-related mortality, with metastasis being a key prognostic factor. CXCR4,CD133 and EpCAM are involved in CRC adhesion, migration, and invasion. CRISPR/Cas9 enables targeted investigation of these molecules as potential therapeutic targets.
Methods: Five original studies examining CXCR4,CD133 and EpCAM in CRC were comparatively analyzed
Results: An increased CD133⁺CXCR4⁺ population was associated with metastasis and poor prognosis. Targeting CXCR4,CD133 and EpCAM reduced cellular adhesion, migration and invasion. SDF-1/CXCR4 activation enhanced cancer-cell adhesion to endothelial cells
Conclusion: CXCR4,CD133 and EpCAM are promising molecular targets for limiting CRC metastasis. CRISPR/Cas9-mediated targeting may reduce aggressive tumor behavior, although further studies are required to establish its specificity and safety
Keywords: Genome editing,CRC,cell adhesion,metastasis,CRISPR/Cas9