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  • Mitomycin and zinc oxide nanoparticle effect on p53 and endothelial growth factor VEGF genes expression in MCF-7 Breast cancer cell line

  • Elham Siasi,1,* Maedeh Cheghini,2 Maryam Bikhof Torbati,3
    1. Department of Genetic, School of science, NT.C., Islamic Azad University, Tehran, Iran
    2. Department of Genetic, School of science, NT.C., Islamic Azad University, Tehran, Iran.
    3. Department of Genetic, School of science, Yadegare-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran


  • Introduction: Breast cancer is one of the most common invasive cancers affecting women worldwide. Zinc oxide nanoparticles, known for their high biocompatibility and have gained significant attention in cancer treatment. Mitomycin C (MMC), a potent antitumor and antibiotic drug, has a broad spectrum of activity against various cancers, its systemic administration often leads to adverse side effects. Aim of this study was identification mitomycin and zinc oxide nanoparticle effect on p53 and endothelial growth factor VEGF genes expression in MCF-7 Breast cancer cell line.
  • Methods: In this study Zinc oxide nanoparticles were synthesized using L-glutamic acid as a green and biocompatible capping agent. Subsequently, MMC was loaded onto the nanoparticles, achieving a high loading percentage of 8.3% and an encapsulation efficiency of 98.8%. The successful synthesis and drug loading were confirmed through Fourier Transform Infrared spectroscopy. To evaluate the cytotoxicity of the nanomedicine, MTT assays were conducted. Differential expression of p53 and endothelial growth factor VEGF genes in MCF-7 Breast cancer cell line were determined by Real-time PCR. Data was statistical analyzed.
  • Results: The results indicated that the nanomedicine exhibited higher toxicity compared to free MMC. Flow cytometry analysis revealed that the nanomedicine induced apoptosis and cell cycle arrest in the G1 phase. Real-time PCR analysis confirmed the upregulation of tumor suppressor gene p53 and vascular endothelial growth factor (VEGF) expression levels in MCF-7 Breast cancer cell line when treatment with mitomycin and zinc oxide nanoparticles (p<0.001).
  • Conclusion: This study results shown that mitomycin and zinc oxide nanoparticles could be had potential effect in MCF-7 cell line of breast cancer toxicity and significant changes in the expression of key cancer-related genes. Therefore, could be enhanced therapeutic applications in cancer treatment. Additional research for confirming these results was recommended.
  • Keywords: mitomycin, zinc oxide nanoparticles, gene expression, breast cancer cell line.

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