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  • Global and Regional DNA methylation of MMP2 and expression of DNA Methyltransferase in gastric adenocarcinoma

  • Sepideh Papi,1,*
    1. Clinical Biochemistry and Student Research Committee, Lorestan University of Medical Sciences.


  • Introduction: The relationship between the expression level of matrix metalloproteinase -2 (MMP2) And epigenetic changes in the pathogenesis of gastric Adenocarcinoma (GA) are largely unknown . We express communication MMP2 with its promoter methylation and status Global methylation DNA Genomics as well gene expression (DNMTs) We investigated DNA methyl transferases in patients with gastric Adenocarcinoma.
  • Methods: Methylation pattern ofMMP-2 promoterin 24 gastric adenocarcinoma tissues and 24 adjacent non-tumor tissues by Methylation-specific PCR (MSP) , and gene expression levels MMP2 in the samples with the method We checked real-time PCR . Also levels global 5-methyl cytosine genomics and gene expression DNMTs Make order using the method ELISA And Real-time PCR we investigated.
  • Results: We found that the reduction of methylation in this particular region of the promoter MMP2 significantly by increasing MMP2 gene expression in patients Adenocarcinoma of the stomach it is related . Also, the amount of 5-methylcytosine in gastric adenocarcinoma (GA) patients Significantly decreased and in addition, the level of expression DNMT1 , DNMT3A , and 3B in patients GA Increased. Mean expression DNMT(1/3A/3B) It had a positive correlation with the tumor area . Also, decreased methylation of MMP2 As the risk increases Adenocarcinoma of the stomach it is. related The analysis of our results showed that the level of MMP2 gene expression in Helicobacter pylori infection has increased significantly compared to the level of MMP2 gene expression in samples without Helicobacter pylori infection (P=0.006) .
  • Conclusion: Our study demonstrated that global methylation DNA and epigenetic activation MMP2 with danger GA It is related that Helicobacter pylori infection with a decrease in the methylation of the whole genome and a decrease in the methylation of the promoter of the MMP2 gene are involved in increasing the incidence of gastric adenocarcinoma. Our data provide molecular mechanistic insight into increased epigenetic activity MMP2 in patients Adenocarcinoma of the stomach provides that it may be a key tumor marker for pathogenesis Gastric adenocarcinoma is related.
  • Keywords: Gastritis Adenocarcinoma, Matrix metalloproteinas-2, Global DNA methylation, DNA methyltransferase,

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