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  • Comparison of mTOR gene expression in the kidney tissue of rats treated with DMBA and an aqueous extract of Centella asiatica

  • Zahra Teymouri,1,* Seyed Hamid Jamaldini Ezabady,2 Zahra Tahmasebi Fard,3
    1. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
    2. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
    3. Associate Professor of Cell and Molecular Biology, Islamic Azad University, Rudehen Branch


  • Introduction: 7,12-Dimethylbenz[a]anthracene (DMBA) is a carcinogenic compound whose exposure may induce molecular alterations in animal tissues. The mTOR gene plays a key role in regulating cell growth and proliferation; therefore, examining its expression may provide insight into how kidney tissue responds to different exposures. This study aimed to compare mTOR gene expression in the kidney tissue of rats treated with DMBA and rats treated with an aqueous extract of Centella asiatica.
  • Methods: This report compares two groups from an animal study. The first group received DMBA at a dose of 10 mg/kg. The second group received only an aqueous extract of C. asiatica at a concentration of 475 mg/mL, administered once by gavage. During the five-week study period, one animal from each group was euthanized each week, and kidney tissue samples were collected. mTOR gene expression in the samples was measured using real-time PCR.
  • Results: The gene expression plots showed a decline in mTOR expression over the five-week study period. Direct comparison of the two groups revealed significantly lower mTOR expression in the extract-treated group than in the DMBA-treated group (p = 0.0001).
  • Conclusion: Comparing these two groups may reveal differences in mTOR expression between rats treated with DMBA and those given the extract alone. The findings may suggest that the extract contributes to the reduction in mTOR expression, which also appears to decline under carcinogenic conditions.
  • Keywords: DMBA; Centella asiatica; mTOR; kidney tissue; real-time PCR

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