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  • Preliminary Assessment of the Effects of Chicory-Derived Inulin on the Viability of Human Renal Carcinoma ACHN Cells

  • Fatemeh Abolmashadi,1,* Mahdi Moridi Farimani,2
    1. Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
    2. Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran


  • Introduction: Inulin is a naturally occurring fructan with established prebiotic properties and potential applications in functional foods and biomedical research. However, its direct effects on human renal carcinoma cells remain poorly characterized. This study aimed to provide a preliminary assessment of the effects of chicory-derived inulin (Cichorium intybus L.) on the viability of human renal carcinoma ACHN cells, establishing an initial experimental basis for further investigation.
  • Methods: ACHN cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum and maintained at 37°C in a humidified atmosphere containing 5% CO₂. Cells were seeded into 96-well plates at a density of 10,000 cells per well and incubated for 24 h to allow attachment. Subsequently, cells were exposed to seven concentrations of chicory-derived inulin (15–960 µM) for 24 and 48 h. Cell viability was assessed using the MTT assay with a final MTT concentration of 0.5 mg/mL. Absorbance was measured at 570 nm, and viability was calculated relative to untreated control cells, with blank wells used for background correction.
  • Results: Inulin treatment was associated with a concentration- and time-dependent reduction in ACHN cell viability. After 24 h, cell viability decreased from 97.01% at the lowest tested concentration to 85.93% at the highest concentration. After 48 h, the corresponding values were 93.98% and 79.50%, respectively. At the highest concentration, the reduction in viability relative to the untreated control was 14.07% after 24 h and 20.50% after 48 h. These findings suggest that prolonged exposure to chicory-derived inulin was associated with a greater reduction in cellular metabolic activity. However, statistical significance has not yet been established.
  • Conclusion: Chicory-derived inulin was associated with a modest reduction in ACHN cell viability, with a more pronounced effect after 48 h of exposure. Further experimental validation is needed to confirm these preliminary findings and establish their biological significance.
  • Keywords: Inulin; Cichorium intybus; ACHN cells; Cell viability; MTT assay

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